Induction of somatic embryogenesis and plant regeneration of sugarcane cv. RB925345 from somatic embryos

Rodrigo de Oliveira Almeida, Sérgio Delmar dos Anjos e Silva, Leonardo Ferreira Dutra


The aim of this study was to establish a protocol for induction of somatic embryogenesis and regeneration of somatic embryos of sugarcane (cultivar RB925345). Best results was obtained using concentration of 13.5 µM of 2,4-dichlorophenoxyacetic acid, 45 days of incubation on induction medium, one subculture and 15 days of incubation on regeneration medium, providing 47.5% of embryogenic calli and 38.9% of plants regenerated. Adjustments are necessary to this protocol to improve the embryogenic calli production rate and regeneration of somatic embryos.


Saccharum spp.; 2,4-dichlorophenoxyacetic acid; plant tissue culture; somatic embryogenesis

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