Crioconservação de sementes de seis acessos de maracujazeiro

Abstract

The cryoconservation has become an interesting alternative for the conservation of germplasm. Researched the seed conservation of passion fruit under two environments: laboratory (25ºC) and liquid nitrogen (-196ºC). Six accesses from IAC germplasm were studied: Passiflora serrato-digitata L., P. nitida Kunth. and four P. edulis Sims. genotypes (yellow, purple, small-purple, rose-apple). Seeds from the mature fruits were processed and evaluated in different moisture contents. Each treatment was composed of 400 seeds in four repetitions of 50 units each. The seed cryoconservation effects were evaluated by standard germination test (STG) and by the following tests of vigor: first counting of the STG (%), to the 7 days, length of seedling (cm) and emergency (%), to the 30 days. It was verified that the cryoconservation significantly affected the seeds quality of the P. edulis Sims. For this species, occurred improvement in the seeds germination and the seedlings emergency of yellow and rose-apple genotypes. For P. serrata-digitata seeds, similar results were 14 obtained to two treatments, showing germination below of 12%. Already, P. nitida seeds had the poorest % germination, independent of moisture contents. For these two last species will necessity of other searches to test cryprotectors for better seed conservations in ultra-low temperatures.