Identification of allyl isothiocyanate in crambe by HPLC

Abstract

The identification of molecules present in plants by chromatographic techniques such as HPLC, can bring promising alternative to the control of plant diseases, since brassicas have glucosinolates, which act in plant defense. The objective of this work was to verify the presence of allyl isothiocyanate by HPLC (High Performance Liquid Chromatography Reverse Phase), using different methods of extraction. The plant material was leaves of Crambe abyssinica, dried at 45 °C for 48 h. The extraction methods used consisted of 200 mg L^-1 of plant material for each method: infusion, aqueous solution, and cetonic, hydroalcoholic, methanol, hexane and chloroform solutions. The identification and quantification were by HPLC on C18 column UV at 244 nm. The experiment consisted of seven treatments with three replicates using the Tukey p <0.05 test. The solvents methanol and hydroalcoholic presented high affinity to molecules of allyl isothiocyanate, and methanol was the best solvent for the extraction, with a concentration of 133.88 mAU. The allyl isothiocyanate was identified at the time of 4.29 min and 4.35 min for methanol and hydroalcoholic extract respectively.