Micropropagation of candeia, native species from brazilian cerrado

Abstract

Candeia (Eremanthus elytropappus) is a native species from Brazilian Cerrado and presenting economic potential for the timber and pharmaceutical industry. However, the ex vitro germination rate of the seeds is considered low. In this context, the objective was to establish a protocol for in vitro germination and multiplication, aiming at the rapid multiplication of the species. For the in vitro germination medium were tested WPM; ½ WPM; ¼ WPM; MS; MS ½ and ¼ MS. In addition, Were Also tested GA₃ (0.0; 0.2; 0.4; 0.6; 0.8 mg L^-1) and pH levels (4.8, 5.8 and 6.8) in ¼ WPM culture medium. For in vitro multiplication, stem segments were inoculated in ¼ WPM supplemented with BAP (0.0, 1.0, 2.0, 3.0, 4.0 and 5.0 mg L^-1). Shoots obtained in vitro were individually and inoculated in WPM ¼ culture, supplemented with IBA (0.0, 1.0, 2.0, 3.0, 4.0 mg L^-1) and 1.5 g L^-1 of activated carbon for the in vitro rooting. The shoots were transferred to plastic tubes containing Plantmax^® for acclimatization. The more efficient culture medium for in vitro germination is ¼ WPM supplemented with 0.56 mg L^-1 GA₃ and pH 4.8. For multiplication must be used ¼ WPM medium supplemented with 2.8 mg L^-1 BAP and 3.1 mg L^-1 IBA. The plants showed 70% of ex vitro acclimatization.