CULTURE MEDIUM AND GROWTH REGULATORS IN THE MICROPROPAGATION OF GRAPEVINE ROOTSTOCKS

Abstract

The micropropagation of grapevine is used, mainly, for the obtaining uniforms plants, virus-free and in a short space of time. With the objective to improve techniques of grapevine rootstocks micropropagation (Vitis sp.), nodal segments with 2,5 cm length and 2 axillary’s buds, deriving by plants in vitro had been excised and inoculated in different culture medium, supplemented with four concentrations of cinetina and four of 6-benzilaminopurin (BAP), in all possible combinations. The pH was adjusted for 6,4 (DSD1) and 5,8 (MS), before the addition 6 g cm^-3 of agar and the sterilization by 121 °C and 1 atm per 20 min. After the inoculation, the explants had been transferred to growth room the 25 ± 2 °C, irradiance of 35  mol m^-2 s^-1 and fotoperíodo of 16 h, where had remained in these conditions per 70 days. The used experimental delineation was casualizated, using 4 repetitions and 3 tubes contends onde explant each. Bigger buds’s number of ‘Kobber 5BB' was verified in culture medium Kc. For the rootstock ‘VR043-43' the culture medium more adjusted in the multiplication in vitro was DSD1. Better resulted in the ‘R110’ micropropagation and little formation of callus had been observed in the absence of growth regulators in the culture medium.