ESTABLISHMENT AND GROWTH OF LIPPIA GRACILIS SCHAUER CULTIVATED UNDER DIFFERENT IN VITRO CONDITIONS

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DOI:

https://doi.org/10.18188/sap.v21i4.30208

Resumen

The objective of this study was to evaluate the explant type, its position and the culture medium used for the establishment and in vitro culture of Lippia gracilis. For the establishment experiment, the treatments were: two types of explants (apical and nodal) taken from two cultivation environments (field and greenhouse), and inoculated in two types of culture medium (MS and MS/2). Half of the salt concentration and the use of explants from a greenhouse reduced the rates of contamination, oxidation, and necrosis in the in vitro establishment of Lippia gracilis. In order to verify the influence of the explant position, the treatments consisted of explants horizontally or vertically inoculated. For the evaluation of sucrose in the culture medium, the concentrations of 7.5; 15; 30 and 60 g L-1 were tested, while for salt concentration, the treatments consisted of 4 different salt concentrations: ¼ MS; ½ MS; MS and 2MS. The sucrose concentration of 30 g L-1 and ½ MS culture medium showed the best growth results for the species. Although the production of dry matter was reduced in this concentration of salts, the plantlets were more vigorous to proceed to acclimatization. Therefore, the inoculation of explants in a horizontal position in ½ MS culture medium, supplemented with 30 g L-1 of sucrose is recommended for in vitro cultivation of L. gracilis.

 Keywords: Carbohydrates, culture medium, micropropagation, salts, tissue culture.

Biografía del autor/a

Luiz Eduardo Santos Lazzarini, Universidade Federal de Lavras - UFLA

Engenheiro Agrônomo formado pela Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), mestre em Zootecnia pela Universidade Federal de Minas Gerais (UFMG) e Doutor em Agronomia / Fitotecnia pela Universidade Federal de Lavras (UFLA). Atualmente é professor substituto na Universidade Federal do Rio Grande do Norte.

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Publicado

30-12-2022

Cómo citar

LAZZARINI, L. E. S.; PACHECO, F. V.; SANTIAGO, A. C.; COELHO, A. D.; BERTOLUCCI, S. K. V.; PINTO, J. E. B. P. ESTABLISHMENT AND GROWTH OF LIPPIA GRACILIS SCHAUER CULTIVATED UNDER DIFFERENT IN VITRO CONDITIONS. Scientia Agraria Paranaensis, [S. l.], v. 21, p. 362–370, 2022. DOI: 10.18188/sap.v21i4.30208. Disponível em: https://e-revista.unioeste.br/index.php/scientiaagraria/article/view/30208. Acesso em: 10 may. 2025.

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Artigos Científicos